MOLECULAR DETECTION OF FUSOBACTERIUM NUCLEATUM IN IN KAZAKHSTANY PATIENTS WITH COLORECTAL CANCER
Main Article Content
Authors
B.A. Kurentay
LLP «National Center for Biotechnology», Korgalzhyn highway 13/5, Astana, Kazakhstan, 010000
A.D. Gusmaulemova
LLP «National Center for Biotechnology», Korgalzhyn highway 13/5, Astana, Kazakhstan, 010000
T.A. Utupov
LLP «National Center for Biotechnology», Korgalzhyn highway 13/5, Astana, Kazakhstan, 010000
D.N. Auganova
LLP «National Center for Biotechnology», Korgalzhyn highway 13/5, Astana, Kazakhstan, 010000
P.V. Tarlykov
LLP «National Center for Biotechnology», Korgalzhyn highway 13/5, Astana, Kazakhstan, 010000
S.S. Khamzina
LLP « National Scientific Cancer Center », Kerey, Zhanibek Khandar str., house 3, Astana, Kazakhstan, 010000
M.A. Mamlin
LLP « National Scientific Cancer Center », Kerey, Zhanibek Khandar str., house 3, Astana, Kazakhstan, 010000
A.M. Kozhakhmetov
Nazarbayev University School of Medicine, Kerey, Zhanibek Khandar str., house 5/1, Astana, Kazakhstan, 010000
S.B. Shalekenov
LLP « National Scientific Cancer Center », Kerey, Zhanibek Khandar str., house 3, Astana, Kazakhstan, 010000
G.N. Kulmambetova
LLP «National Center for Biotechnology», Korgalzhyn highway 13/5, Astana, Kazakhstan, 010000
Abstract
Colorectal cancer is the leading cause of cancer death worldwide. Fusobacterium nucleatum is thought to play an important role in the development of colorectal cancer. F. nucleatum there are many studies that prove that has a great influence on the oncogenesis of colorectal cancer, but the participation of the bacterium in this process remains unclear. The goal of our study is to find out in colorectal cancer tissue F. nucleatum was the definition. Biomaterials from 43 colorectal cancer patients from the National scientific cancer center were analyzed. To detect bacteria, DNA was extracted from the biomaterial and culture inoculation was carried out in parallel. F. nucleatum detection was performed by two methods using Matrix activated laser microbiological desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and quantitative real-time PCR detection. As a result, MALDI determined 14.58%, and qPCR detected 46.51% of bacteria. Thus, F. nucleatum can be a biomarker of colorectal cancer risk.
Keywords
Fusobacterium nucleatum, колоректальный рак, детекция, кПЦР, MALDI, опухоль
Article Details
References
World Health Organization. Colorectal cancer // Retrieved from:
URL 20is%20the%20second,and%20mortality%20rates%20were%20observed.
Mauyenova D., Zhadykova Y., Khozhayev A., Turebayev D., Kulmirzayeva D., Urazova S., Nurtazinova G., Kuandykov Y., Amanshayeva A., Sakhanov S., Bukeyeva Z., Openko V., Orazbayev S., Kozhakhmetov S., Bilyalova Z., Igissinova G., Alireza Mosavi Jarrahi A., Igissinov N. Trends of Colorectal Cancer Incidence in Kazakhstan // Asian Pac J Cancer Prev. - 2021. - Vol. 22, No. 10. - P. 3405. doi: 10.31557/APJCP.2021.22.10.3405. PMID: 34714614; PMCID: PMC8858261.
Amankulov J., Kaidarova D., Zholdybay Z., Zagurovskaya M., Baltabekov N., Gabdullina M., Ainakulova A., Toleshbayev D., Panina A., Satbayeva E., Kalieva Z. Colorectal Cancer Screening with Computed Tomography Colonography: Single Region Experience in Kazakhstan // Clin Endosc. - 2022. Vol. 55, No. 1. - P. 101-112. doi: 10.5946/ce.2021.066. Epub 2021 Jul 15. PMID: 34265195; PMCID: PMC8831409.
Kaminski M.F., Robertson D.J., Senore C., Rex D.K. Optimizing the Quality of Colorectal Cancer Screening Worldwide // Gastroenterology. - 2020. Vol. 158. - P. 404. doi: 10.1053/j.gastro.2019.11.026. PMID: 31759062
Sánchez-Alcoholado L., Ramos-Molina B., Otero A., Laborda-Illanes A., Ordóñez R., Medina J.A., Gómez-Millán J., Queipo-Ortuño M.I. The Role of the Gut Microbiome in Colorectal Cancer Development and Therapy Response // Cancers (Basel). - 2020. - Vol. 29, No. 6. - P. 1406. doi: 10.3390/cancers12061406. PMID: 32486066; PMCID: PMC7352899.
Castellarin M., Warren R.L., Freeman J.D., et al. Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma // Genome Research. - 2012. Vol. 22, No. 2. - P. 299–306. doi: 10.1101/gr.126516.111. PMID: 22009989; PMCID: PMC3266037
Kostic A.D., Gevers D., Pedamallu C.S., Michaud M., Duke F., Earl A.M., Ojesina A.I., Jung J., Bass A.J., Tabernero J., Baselga J., Liu C., Shivdasani R.A., Ogino S., Birren B.W., Huttenhower C., Garrett W.S., Meyerson M. Genomic analysis identifes association of Fusobacterium with colorectal carcinoma // Genome Res. - 2012. Vol. 22, No. 2. - P. 292–298. doi: 10.1101/gr.126573.111. PMID: 22009990; PMCID: PMC3266036.
Rubinstein M.R., Wang X., Liu W., Hao Y,. Cai G., Han Y.W. Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin // Cell Host Microbe. - 2013. Vol. 14, No. 2. - P. 195–206. doi: 10.1016/j.chom.2013.07.012. PMID: 23954158; PMCID: PMC3770529.
Rubinstein M.R., Baik J.E., Lagana S.M., Han R.P., Raab W.J., Sahoo D., Dalerba P., Wang T.C., Han Y.W. Fusobacterium nucleatum promotes colorectal cancer by inducing Wnt/β-catenin modulator Annexin A1 // EMBO Rep. - 2019. Vol. 20, No. 4. - P. e47638. doi: 10.15252/embr.201847638. Epub 2019 Mar 4. PMID: 30833345; PMCID: PMC6446206.
Kostic A.D., Chun E., Robertson L., Glickman J.N., Gallini C.A., Michaud M., Clancy T.E., Chung D.C., Lochhead P., Hold G.L., ElOmar E.M., Brenner D., Fuchs C.S., Meyerson M., Garrett W.S. Fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor-immune microenvironment // Cell Host Microbe. - 2013. Vol. 14, No. 2. - P. 207–215. doi: 10.1016/j.chom.2013.07.007. PMID: 23954159; PMCID: PMC3772512.
Mima K., Nishihara R., Qian Z.R., Cao Y., Sukawa Y., Nowak J.A., Yang J., Dou R., Masugi Y., Song M., Kostic A.D., Giannakis M., Bullman S., Milner D.A., Baba H., Giovannucci E.L., Garraway L.A., Freeman G.J., Dranof G., Garrett W.S., Huttenhower C., Meyerson M., Meyerhardt J.A., Chan A.T., Fuchs C.S., Ogino S. Fusobacterium nucleatum in colorectal carcinoma tissue and patient prognosis // Gut. - 2016. Vol. 65, No. 12. - P. 1973–1980. doi: 10.1136/gutjnl-2015-310101. PMID: 26311717; PMCID: PMC4769120.
Rubinstein M.R., Wang X., Liu W., et al. Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/beta-catenin signaling via its FadA adhesin // Cell Host Microbe. - 2013. - Vol. 14, No. 2. - P. 195–206. doi: 10.1016/j.chom.2013.07.012. PMID: 23954158; PMCID: PMC3770529.
Rubinstein M.R., Baik J.E., Lagana S.M., Han R.P., Raab W.J., Sahoo D., Dalerba P., Wang T.C., Han Y.W. Fusobacterium nucleatum promotes colorectal cancer by inducing Wnt/β-catenin modulator Annexin A1 // EMBO Rep. - 2019. Vol. 20. - P. e47638. doi: 10.15252/embr.201847638. PMID: 30833345; PMCID: PMC6446206.
Brennan C.A., Garrett W.S. Fusobacterium nucleatum—symbiont, opportunist and oncobacterium // Nat. Rev. Genet. - 2018. Vol. 17. - P. 156–166. doi: 10.1038/s41579-018-0129-6. PMID: 30546113; PMCID: PMC6589823.
Brennan C.A., Garrett W.S. Fusobacterium nucleatum – symbiont, opportunist and oncobacterium // Nat Rev Microbiol. - 2019. Vol. 17, No. 3. - P. 156–166. doi: 10.1038/s41579-018-0129-6. PMID: 30546113; PMCID: PMC6589823.
Bradshaw D.J., Marsh P.D., Watson G.K., Allison C. Role of Fusobacterium nucleatum and coaggregation in anaerobe survival in planktonic and bioflm oral microbial communities during aeration // Infect Immunol. - 1998. Vol. 66, No. 10. - P. 4729–4732. doi: 10.1128/IAI.66.10.4729-4732.1998. PMID: 9746571; PMCID: PMC10858
Han Y.W., Fardini Y., Chen C., Iacampo K.G., Peraino V.A., Shamonki J.M., Redline R.W. Term stillbirth caused by oral Fusobacterium nucleatum // Obstet Gynecol. - 2010. Vol. 115, No. 2. - P. 442–445. doi: 10.1097/AOG.0b013e3181cb9955. PMID: 20093874; PMCID: PMC3004155.
Kai A., Cooke F., Antoun N., Siddharthan C., Sule O. A rare presentation of ventriculitis and brain abscess caused by Fusobacterium nucleatum // J Med Microbiol. - 2008. Vol. 57, No. 5. - P. 668–671. doi: 10.1099/jmm.0.47710-0. PMID: 18436604.
Yoneda M., Kato S., Mawatari H., Kirikoshi H., Imajo K., Fujita K., Endo H., Takahashi H., Inamori M., Kobayashi N., Kubota K., Saito S., Tohnai I., Watanuki K., Wada K., Maeda S., Nakajima A. Liver abscess caused by periodontal bacterial infection with Fusobacterium necrophorum // Hepatol Res. - 2011. Vol. 41, No. 2. - P. 194–196. doi: 10.1159/000356821. PMID: 24348321; PMCID: PMC3843899
Han Y.W., Redline R.W., Li M., Yin L., Hill G.B., McCormick T.S. Fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth // Infect Immunol. - 2004. Vol. 72, No. 4. - P. 2272–2279. doi: 10.1128/IAI.72.4.2272-2279.2004. PMID: 15039352; PMCID: PMC375172.
Strauss J., Kaplan G.G., Beck P.L., Rioux K., Panaccione R., Devinney R., Lynch T., Allen-Vercoe E. Invasive potential of gut mucosa-derived Fusobacterium nucleatum positively correlates with IBD status of the host // Infamm Bowel Dis. - 2011. Vol. 17, No. 9. - P. 1971– 1978. doi: 10.1002/ibd.21606. PMID: 21830275.
Liu H., Hong X.L., Sun T.T., Huang X.W., Wang J.L., Xiong H. Fusobacterium nucleatum exacerbates colitis by damaging epithelial barriers and inducing aberrant infammation // J Dig Dis. - 2020. Vol. 21, No. 7. - P. 385–398. doi: 10.1111/1751-2980.12909. PMID: 32441482.
Li R., Shen J., Xu Y. Fusobacterium nucleatum and Colorectal Cancer // Infect Drug Resist. - 2022. Vol. 17, No. 15. - P. 1115-1120. doi: 10.2147/IDR.S357922. PMID: 35321079; PMCID: PMC8937307.
Ahmad Kendong S.M., Raja Ali R.A., Nawawi K.N.M., Ahmad H.F., Mokhtar N.M. Gut dysbiosis and intestinal barrier dysfunction: potential explanation for early-onset colorectal cancer // Front Cell Infect Microbiol. - 2021. Vol. 11. - P. e744606. doi: 10.3389/fcimb.2021.744606. PMID: 34966694; PMCID: PMC8710575.
Nasiri H., Forouzandeh M., Rasaee M.J., Rahbarizadeh F. Modified salting-out method: high-yield, high-quality genomic DNA extraction from whole blood using laundry detergent // J Clin Lab Anal. – 2005. Vol. 19, No. 6. – P. 229-32. doi: 10.1002/jcla.20083. PMID: 16302208; PMCID: PMC6808030.
Mima K., Sukawa Y., Nishihara R., Qian Z.R., Yamauchi M., Inamura K., Kim S.A., Masuda A., Nowak J.A., Nosho K., Kostic A.D., Giannakis M., Watanabe H., Bullman S., Milner D.A., Harris C.C., Giovannucci E., Garraway L.A., Freeman G.J., Dranof G., Chan A.T., Garrett W.S., Huttenhower C., Fuchs C.S., Ogino S. Fusobacterium nucleatum and T cells in colorectal carcinoma // JAMA Oncol. - 2015. Vol. 1, No.5. - P. 653–661. doi: 10.1001/jamaoncol.2015.1377. PMID: 26181352; PMCID: PMC4537376.
Mima K., Cao Y., Chan A. T., Qian Z. R., Nowak J. A., Masugi Y., Ogino S. Fusobacterium nucleatum in colorectal carcinoma tissue according to tumor location // Clinical and Translational Gastroenterology. - 2016. Vol. 7, No. 11. - P. e200. doi: 10.1038/ctg.2016.53. PMID: 27811909; PMCID: PMC5543402.
Abd El-Aziz N.K., Gharib A.A., Mohamed E.A.A., Hussein A.H. Real-time PCR versus MALDI-TOF MS and culture-based techniques for diagnosis of bloodstream and pyogenic infections in humans and animals // J Appl Microbiol. – 2021. Vol. 130, No. 5. – P. 1630-1644. doi: 10.1111/jam.14862. Epub 2020 Oct 18. PMID: 33073430.
Li Y.Y., Ge Q.X., Cao J., Zhou Y.J., Du Y.L., Shen B., Nie Y.Q. Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients // World Journal of Gastroenterology. - 2016. Vol. 22, No. 22. - P. e3227. doi: 10.3748/wjg.v22.i11.3227. PMID: 27004000; PMCID: PMC4789998.
Ito M., Kanno S., Nosho K., Sukawa Y., Mitsuhashi K., Kurihara H., Shinomura Y. Association of Fusobacterium nucleatum with clinical and molecular features in colorectal serrated pathway // International Journal of Cancer. - 2015. Vol. 137, No. 6. - P. 1258–1268. doi: 10.1002/ijc.29488. PMID: 25703934.
Yamaoka Y., Suehiro Y., Hashimoto S., Hoshida T., Fujimoto M., Watanabe M., Yamasaki T. Fusobacterium nucleatum as a prognostic marker of colorectal cancer in a Japanese population // Journal of Gastroenterology. - 2018. Vol. 53, No. 4. - P. 517–524. doi: 10.1007/s00535-017-1382-6. PMID: 28823057.
Kostic A. D., Chun E., Robertson L., Glickman J. N., Gallini C. A., Michaud M., Garrett, W. S. Fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor‐ immune microenvironment // Cell Host & Microbe. - 2013. Vol. 14, No. 2. - P. 207–215. doi: 10.1016/j.chom.2013.07.007. PMID: 23954159; PMCID: PMC3772512.
Tahara T, Yamamoto E, Suzuki H, Maruyama R, Chung W, Garriga J, Jelinek J, Yamano H, Sugai T, An B, Shureiqi I, Toyota M, Kondo Y, Estécio M, and Issa J. Fusobacterium in colonic flora and molecular features of colorectal carcinoma. Cancer Res. 2014 March 1; 74(5): 1311–1318. doi:10.1158/0008-5472.CAN-13-1865.