ENZYME-LINKED IMMUNOSORBENT ASSAY BASED ON THE RECOMBINANT VP1 ANTIGEN FOR SEROLOGICAL DIAGNOSIS OF FMD
Main Article Content
Authors
K.N. Mukantayev
National Center for Biotechnology, Valikhanov str., 13/1, Astana, 010000, Kazakhstan
A.B. Shustov
National Center for Biotechnology, Valikhanov str., 13/1, Astana, 010000, Kazakhstan
I. Sydyknabi
National Center for Biotechnology, Valikhanov str., 13/1, Astana, 010000, Kazakhstan
B. Inerbay
National Center for Biotechnology, Valikhanov str., 13/1, Astana, 010000, Kazakhstan
A. Bigalyeva
National Center for Biotechnology, Valikhanov str., 13/1, Astana, 010000, Kazakhstan
K.K. Mukanov
National Center for Biotechnology, Valikhanov str., 13/1, Astana, 010000, Kazakhstan
Abstract
Diagnostic tests play an important role in prophylactic and quarantine events against FMD. Therefore, nowadays development of effective methods in diagnostics of diseases is considered as a main problem. Properties of the diagnostic test-systems depend on quality of the used antibodies and antigens. Use of recombinant antigens can improve the diagnostics methods of diseases. The purpose of this study is purification and use of recombinant VP1 antigen for diagnosis of FMD by Enzyme-linked immunosorbent assay.
For purification of recombinant antigens there was used metal-affinity chromatography with the column HisTrapTM FF crude (GE Healthcare, Sweden). For the definition of diagnostic properties of antigens by ELISA there were used 186 serum samples of vaccinated animals and 64 serum samples of unvaccinated animals.
There have been determined diagnostic properties of VP1 O and VP1 Asia-1 recombinant antigens by means of ELISA. The data obtained showed high effectiveness of the used purification method and suitability of the purified antigens for serological diagnosis of FMD by means of indirect ELISA.
Keywords
virus, food-and-mouth diseases, structural antigens, recombinant protein, enzyme-linked immunosorbent assay
Article Details
References
Kitching R.P. Foot-and-mouth disease: current world situation. Vaccine, 1999, vol. 17, рр. 1772-1774. doi: 10194838.
Ferris N.P., Dawson M. Routine application of enzymelinked immunosorbent assay in comparison with complement fixation for the diagnosis of foot-and-mouth and swine vesicular diseases. Veterinary Microbiology, 1988, vol. 16, рр. 201-209. doi: 3376418
Reid S. M., Grierson S. S., Ferris N. P. Evaluation of automated RT-PCR to accelerate the laboratory diagnosis of foot-and-mouth disease virus. Journal of Virology Methods, 2003, vol. 107, рр. 129-139. doi: 12505626.
Houhui Song, Weihuan Fang, Zhiliang Wang, Dongxia Zheng, Jian Du, Hong Li, Yong Li, Bingsheng Qiu. Detection of foot-and-mouth virus antibodies using a purified protein from the high-level expression of codon-optimized, foot-and-mouth disease virus complex epitopes in Escherichia coli. Biotechnology Letters, 2004, vol. 26, рр. 1277-1281. doi: 15483387.
Hamblin C., Barnett I.T.R., Hedger R.S. A new enzymelinked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. Journal of Immunology Methods, 1986, vol. 93, рр. 115-121. doi: 3021854.
Denac H., Moser C., Tratschin J.-D., Hofmann M.A. An indirect ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus using recombinant nucleocapsid protein as antigen. Journal of Virology Methods, 1997, vol. 65, рр. 169-181. doi: 9186940.
Moser C., Ruggli N., Tratschin J.D., Hofmann M.A. Detection of antibodies against classical swine fever virus in swine sera by indirect ELISA using recombinant envelope glycoprotein E2. Veterinary Microbiology, 1996, vol. 51, рр. 41-53. doi: 8828121.
De Boer G.F., Boerrigter H.M., Groen J., Osterhaus A.D. Identifi cation of bovine leukemia virus (BLV) infected cattle by complex-trapping-blocking (CTB) ELISA employing monoclonal antibodies directed against BLV-p24. Zentralbl Veterinarmed B, 1987, no. 34, pp. 717-728. doi: 2830742.
Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, vol. 227, pp. 680-685. doi: 5432063.
Bradford M.M. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, vol. 72, pp. 248-254. doi: 942051.
Wenger M., Tratschin J.D., Hofmann M.A. Indirect ELISA using recombinant VP1 capsid protein for the serodifferentiation of foot-and-mouth disease virus infected animals. In: Repor; European Commission for the Control of Foot-and-Mouth Disease. Research Group of the Standing Technical Committee, Borovets (Bulgaria), 5-8 Sep 2000. FAO, Rome (Italy). Animal Production and Health Div, 2000, pp. 222-223.
Keltsieva O.A., Gladilovich V.D., Podolskya E.P. Metal-affine chromatography. Basics and application. Scientific instrumentation, 2013, vol. 23, рр. 74–85.
Ivanov K.I., Ivanov P.A., Timofeev E.K., Efimov V.A., Dorochov J.L. Cloning genes of transport proteins of two strains tobacco mosaic virus and their expression in Escherichia coli cells. Bioorganic chemical, 1994, vol. 20, рр. 751-758. doi: 7993379.
Chien-Der Lee, Yao-Pei Yan, Shu-Mei Liang, Ting-Fang Wang. Production of FMDV virus-like particles by a SUMO fusion protein approach in Escherichia coli. Journal of Biomedical Science, 2009, vol. 16, рр. 69-76. doi:10.1186/1423-0127-16-69.
Mercedes Gareia-Valcarcel, Timothy Doel, Trevor Collen, Martin Ryan, R. Michael E. Parkhouse. Recognition of foot-and-mouth disease virus and its capsid protein VP1 by bovine peripheral T lymphocytes. Journal of General Virology, 1996, vol. 77, рр. 727-735. doi: 8627261.