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A.M. Kokhmetova

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040, Kazakhstan

Z.B. Sapakhova

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040,

A.K. Madenova

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040,

G.T. Yessenbekova

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040,


Wheat rust diseases are a major cause of yield losses of this crop. Yellow (Puccinia striiformis f. sp. tritici) and leaf (Puccinia recondita Desm f. tritici Eriks) rusts are of the most widespread and dangerous diseases of wheat and are the major factor that adversely affects wheat yield and quality and causes considerable economic damage. Use of genetic host resistance is the most effective, economical and environmentally safe method of controlling stripe rust that allow to eliminate the use of fungicides and minimize crop losses from this disease. Due to the threat of development epiphytoties rust disease it is necessary to identify new donors of resistance to yellow and leaf rust and creation on their basis of wheat breeding material. As a result phytopathological assesses susceptibility to rust on infectious background we have selected a number of samples resistant to Puccinia striiformis and Puccinia recondita f. sp. tritici. In the present study, attention was drawn to the part of the effective resistance genes to yellow and leaf rust – Yr5, Yr10 Yr15, as well as gene complex Lr26/Sr31/Yr9/Pm8 and Yr18/Lr34, which were identified in the process of molecular screening of wheat germplasm. Using molecular markers gene Yr5 identified in 1 sample, Yr10 – in 5, Yr15 – in 2, complex of resistance genes Lr26/Sr31/Yr9/Pm8 – in 3 and gene complex Yr18/Lr34 – in 6 samples. The most valuable donors of rust resistance are cultivars Mereke 70 and Akdala with 2 identified rust resistance genes. In cv. Mereke 70 highly efficient genes Yr10 and Yr18/Lr34 and in cv. Dastan Yr10 and Yr15 were identified. The results are used in Kazakhstan to create yellow and leaf rust resistant wheat varieties using MAS breeding. Our results provide an opportunity to move the breeding process in Kazakhstan to a new scientific level through the use of technology Marker Assisted Selection.


wheat, resistance genes, yellow rust, leaf rust, molecular markers

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Official site of FAO (Food and Agriculture Organization – FAO (

Morgounov A., Abugalieva A., Martynov S. 2013a. Effect of Climate Change and Variety on Long-term Variation of Grain Yield and Quality in Winter Wheat in Kazakhstan. Cereal Research Communications, 2013. doi: 10.1556/CRC.2013.0047.

Kokhmetova A., Yessenbekova G., Morgounov A., Ogbonnaya F. The screening of wheat germplasm for resistance to stripe and leaf rust in Kazakhstan using molecular markers. Journal of Life Sciences, USA, 2012, vol. 6, no. 4, рр. 353-362.

FAO sozdala veb-sajt dlja kontrolja za rasprostraneniem rzhavchiny pshenicy (Rust spore) [FAO has created a web site to control the spread of wheat rust (Rust spore)] // URL.

Kokhmetova A., Chen X., Rsaliyev S. Identification of Puccinia striiformis f.sp. tritici. Characterization of wheat cultivars for resistance, and inheritance of resistance to stripe rust in Kazakhstan wheat cultivars. The Asian and Australasian Journal of Plant Science and Biotechnology, 2010, vol. 4, рр. 64-70.

Ziyaev Z.M., Sharma R.C., Nazari K. et al. Improving wheat stripe rust resistance in Central Asia and the Caucasus. Euphytica, 2011, vol. 179, рp. 197-207.

Yahaoui A. Management of yellow rust in Central, Western Asia and Caucasus countries. Newsletter of CIMMYT, 2003, no. 2(5), pp. 113-116.

Zhivotkov L.A., Birjukov S.V., Stepanenko A.Ja. Pshenica [Wheaat]. Ed. L.A.Zhivotkov. Kiev, Urozhaj [Yield], 1989, 319 p.

Wellings C.R., Park R.F. Global perspectives in wheat yellow rust: meeting the challenges of dynamic shifts in pathogen populations. Abstr. of 3-rd Regional Yellow Rust Workshop. Tashkent, Uzbekistan, June 8-11, 2006.

Chen X.M. Epidemiology and control of stripe rust on wheat. Can J Plant Pathol, 2005, vol. 27, pp. 314-337. doi:10.1080/07060660509507230

Kojshibaev M.K., Ponomareva L.A. Vredonosnost' boleznej jarovoj pshenicy s vozdushno-kapel'noj infekciej v Severnom Kazahstane [Disease severity of spring wheat with droplet infection in Northern Kazakhstan]. Vestn. sel'hoz. nauki Kazahstana [Herald of Agricultural Researches in Kazakhstan], 2008, no. 8, pp. 15-19.

Turapin V.P., Bridge V.A. Rust diseases of grain crops in the Republic of Kazakhstan and the fight against them [Rust diseases of cereals in Republic of Kazakhstan]. Almaty, Gylym [Science], 1995, 141 р.

Roelfs A.P., Singh R.P., Saari E.E. Rust Diseases of Wheat: Concept and methods of disease management. Mexico, D.F.: CIMMYT, 1992, 81 p.

Bimb H.P., Johnson R. Breeding resistance to yellow (stripe) rust in wheat. Mexico, DF (Mexico). CIMMYT. Series: CIMMYT Wheat Special Report (WPSR), 1997, no. 41, 20 p.

Kumar J., Singh R.P., Nagarajan S., Sharma A.K. Further evidences on the usefulness of Lr34/Yr18 gene in developing adult plant resistant wheat genotypes. Wheat Inf. Service,1999, no. 89, рр. 23-29.

Chen X., Line R., Leung H. Genome scanning for resistance gene analogs in rice, barley, and wheat by high resolution electrophoresis. Theor. Appl. Genet, 1998, vol. 97, рр. 345-355.

Parlevliet J.E. Strategies for the utilization of partial resistance for the control of cereal rusts. In Simmonds N.W., Rajaram S. (Eds.), Breeding strategies for resistance to the rusts of wheat, Mexico, D.F. CIMMYT, 1988, рр. 48-62.

Caldwel R.M. Breeding for general and/or specific plant disease resistance. In K.W. Finlay and K.W. Shepherd (Eds.) Proc. Int. Wheat Genet. Symp., 3rd, Austr. Acad. Sci., Canberra, Australia. 5-9 Aug. Butterworths, Sydney, 1968, рр. 263-272.

Singh R.P., Huerta-Espino J., William M. Genetics and Breeding for durable resistance to leaf and stripe rust of wheat. Proc. 1-st Central Asia Wheat conf., Kazakhstan, 10-13 June, 2003, рр. 127-132.

McIntosh R.A., Dubcovsky J., Rogers J., Morris C., Appels R., Xia X. Catalogue of gene symbols for wheat: 2010 supplement. URL komugi/genes/macgene 2010.

Yan G.P., Chen X.M., Line R.F., Wellings C.R. Resistance gene analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust have homology with plant disease resistance genes. Theor. Appl. Genet, 2003, vol. 106(4), рр. 636–643. doi: 10.1007/s00122-002-1109-8.

Chen X.M., Moore M.K. and Wood D.A. Epidemiology and control of stripe rusts of wheat and barley in the United States. In "Abstracts of 8th Int. Cong. of Plant Pathol. 2–7 Feb., 2003. Christchurch, New Zealand, vol. 2, рр. 118.

Shi Z.X., Chen X.M., Line R.F., Leung H., Wellings C.R. Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome, 2001, vol. 44, pр. 509-516. doi: 10.1139/gen-44-4-509.

Shao Y.T., Niu Y.C., Zhu L.H., Zhai W.X., Xu S.C., Wu L.R. Identification of an AFLP marker linked to the stripe rust resistance gene Yr10 in wheat. Chinese Science Bulletin, 2001, vol. 46(17), рр. 1466-1468.

Bariana H.S., Hayden M.J., Ahmed N.U., Bell J.A., Sharp P.J., McIntosh R.A. Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Australian Journal of Agricultural Research, 2001, vol. 52(12), рр. 1247–1255. doi:10.1071/AR01040.

Chen, X.M., Moore M., Milus E.A., Long D., Marshall D., Line R.F., and Jackson L. Wheat stripe rust epidemic and races of Puccinia striiformis f. sp. tritici in the United States in 2000. Plant Dis., 2002, vol. 86, рр. 39–46. doi: org/10.1094/PDIS.2002.86.1.39.

Singh R.P., Nelson J.C., Sorells M.E. Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci., 2000, vol. 40(4), рр. 1148-1155. doi:10.2135/cropsci2000.4041148x.

Bespalova L.A., Vasilev A.V., Ablova I.B., Filobok V.A., Hudokormova Zh.N., Davojan R.O., Davojan Je.R., Karlov G.I., Solov'ev A.A., Divashuk M.G., Majer N.K., Dudnikov M.V., Mironenko N.V., Baranova O.A. Primenenie molekuljarnyh markerov v selekcii pshenicy v Krasnodarskom NIISH im. P.P. Lukjanenko [Use of molecular markers in wheat breeding at the Krasnodar Lukyanenko Research Institute of Agriculture]. Vavilovskij zhurnal genetiki i selekcii – Vavilov’s Journal of Genetics and Breeding, 2012, vol. 16. no. 1, pp. 37-43.

Lagudah, E.S., McFadden H., Singh R.P., Huerta-Espino J., Bariana H.S., Spielmeyer W. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet, 2006, vol. 114(1), рр. 21-30. doi: 10.1007/s00122-006-0406-z.

Peterson R.F., Campbell A.B., Hannah A.E. A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Canadian Journal of Research, 1948, vol. 26c(5), рр. 496–500.

Riede C.R., Anderson, J.A. Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci, 1996, vol. 36(4), рр. 905–909. doi:10.2135/cropsci1996.0011183X0036000400015x

Chen X., Soria M. A.., Yan G., Sun J., Dubcovsky J. Development of sequence tagged site and cleaved amplified polymorphic sequence markers for wheat stripe rust resistance gene Yr5. Crop Sci., 2003, vol. 43(6), рр. 2058-2064. doi:10.2135/cropsci2003.2058.

Peng J.H., Fahima T., Röder M.S., Huang Q.Y., Dahan A., Li Y.C., Grama A., Nevo E. High-density molecular map of chromosome region harboring stripe-rust resistance genes YrH52 and Yr15 derived from wild emmer wheat, Triticum dicoccoides. Genetica, 2000, vol. 109(3), рр. 199–210.

Mago R., Spielmeyer W., Lawrence G.J., Lagudah E.S., Ellis J.G., Pryor A. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor. Appl. Genet, 2002, vol. 104(8), рр. 1317-1324. doi: 10.1007/s00122-002-0879-3.

Macer R.C.F. The formal and monosomic genetic analysis of stripe rust (Puccinia striiformis) resistance in wheat. In: IJ. Mackey (ed.) Proc. of 2nd Int. Wheat Genet. Symp. Lund, Sweden 1963. Hereditas Suppl, 1966, vol. 2, рр. 127-142.

Law C.N. Genetic control of yellow rust resistance in T. spelta album. In: Plant Breeding Institute, Cambridge, Annual Report 1975, 1976, рр. 108-109.

Metzger R.J., Silbaugh B.A. Inheritance of resistance to stripe rust and its association with glume colour in Triticum aestivum L. Crop Sci., 1970, vol. 10, рр. 567–568. doi:10.2135/cropsci1970.0011183X001000050035x.

Payne P.I., Holt L.M., Johnson R., Snape J.W. Linkage mapping of four gene loci, Glu-B1, Gli-B1, Rg1and Yr10 on chromosome 1B of bread wheat. Genet Agraria, 1986, vol. 40, рр. 231–242.

Wang L.F., Ma J.X., Zhou R.H., Wang X.M. and Jia J.Z. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, P.I. 178383 (Triticum aestivum L.). Euphytica, 2002(1), vol. 124, рр. 71-73.

Gerechter-Amitai Z.K., Van Silfhout C.H., Grama A., Kleitman F. Yr15: A new gene for resistance to Puccinia striiformis in Triticum dicoccoides sel. G-25. Euphytica, 1989, vol. 43(1-2), рр. 187-190.

McIntosh R.A., Silk J. Cytogenetic studies in wheat XVII. Monosomic analysis and linkage relationships of gene Yr15 for resistance to stripe rust. Euphytica, 1996, vol. 89(3), рр. 395-399.

Disease resistance. Stripe rust. Yr15.Wheat Cap http: