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M.Y. Omasheva

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040, Kazakhstan

S.V. Chekalin

Institute of Botany and Phytointroduction,  45 Timiryazev str., Almaty, 050040, Kazakhstan

N.A. Ryabushkina

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040, Kazakhstan

N.N. Galiakparov

Institute of Plant Biology and Biotechnology, 45 Timiryazev str., Almaty, 050040, Kazakhstan


The mountain regions of Kazakhstan are one of the centers of the origin of wild apples. A set of seven polymorphic microsatellite markers was used to study the biodiversity of apple populations in the Tien Shan region of Kazakhstan, including three populations of Dzhungarian and one population of Tarbagatai. Leaf samples were collected from 120 trees. A set of seven markers and nonspecific sequences, including D8S1132, DYS437, D12S1090, and VIC, 6-FAM, and NED fluorescent labels that were used as adapters were used. A total of 74 alleles were detected with the seven markers. The number of polymorphic alleles ranged from 5 to 14 for GD100 and GD162 loci, respectively, and the average number of alleles per locus was 10.6. The mean value of the effective number of alleles (Ne) per locus was 3.8. The polymorphic information content (PIC) ranged from 0.593 for locus GD100 to 0.779 for locus GD142, and the average was 0,691. The level of expected (He) and observed heterozygosity (Ho) for all of the loci was high and averaged 0.724 and 0.710, respectively. The average level of the Shannon index (I) as a sum of the loci for all of the analyzed samples was 1.62 ± 0.085. The indexes of diversity in the populations ranged from 1.33 ± 0.091 (Krutoe tract) to 1.65 ± 0.092 (the right bank of the Lepsy River) and for the most closely spaced populations. According to Wright statistics, the interpopulation variability accounted for only 3.4% with the remaining variability allocated within each of the M. sieversii populations. A genomic DNA bank of the wild apple populations of Dzhungarian and Tarbagatai has been established.


Malus sieversii, population, genetic diversity, SSR markers, DNA polymorphism, PCR

Article Details


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