IN VITRO CULTURE OF Rhodiola rosea L.

Main Article Content


O.N. Khapilina

National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan

Z.S. Kupeshev

National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan

A.N. Danilova

National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan

R.N. Kalendar

National Center for Biotechnology, Kurgalzhyn road, 13/5, Astana, 010000, Kazakhstan


We present the results of our study on in vitro culture of the valuable medicinal plant Rhodiola rosea (Rhodiola rosea L.). We collected sample plant materials from the Kazakh Altai territory. The essential stages of in vitro cultivation techniques for R. rosea were optimized. We determined the most effective sterilization protocol for different types of explants of Rhodiola and optimized stages of organogenesis and formation of adventitious shoots. We found that aseptic cultures of Rhodiola necessitate the use of multi-stage sterilization protocol using different types of antiseptics. Apexes of shoots and rhizome buds were the most effective explants. High concentration of exogenous cytokinin in the culture medium induced adventitious organogenesis in R. rosea. Maximum adventitious shoots (up to 34 explants) were observed usingMSZ1 medium supplemented with zeatin, indole-3-acetic acid, and gibberellic acidat concentrations of 2, 0.1, and 0.5 mg/L, respectively. The formation of the root system wasdependent on the agar concentration in the medium. Morphometric characteristics were higher in regenerated Rhodiolagrown on a medium containing 0.6% agar.


golden root, Rhodiola rosea L., in vitro culture, explants, adventitious organogenesis, micropropagation

Article Details


OhranaokruzhajushhejsredyiustojchivoerazvitieKazahstana/Statisticheskijsbornik [Environmental protection and sustainable development in Kazakhstan / Statistical Yearbook.Agency of Statistics of RK]. Agentstvo o statistike RK, Astana, 2008, 269 p.

Krajewska-PatanА. Chemical investigations of biotransformedRhodiolarosea callus tissue. Herbapolonica, 2007, vol. 53, pp. 77-87.

Canter P.H., Thomas H., Ernst E. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends in biotechnology, 2005, vol. 23, no. 4, pp.180-185. DOI:10.1016/j.tibtech.2005.02.002.

Reed B., Sarasan M., Kane V. Biodiversity conservation and conservation biotechnology tools. In vitro cell developmental biology plant, 2011, no. 47, pp. 1-4. DOI:10.1007/s11627-010-9337-0.

Tripathi L., Tripathi J.N. Role of biotechnology in medicinal plants. Tropical journal of pharmaceutical research, 2003, vol. 2, no. 2, pp.243-253.

Edison S., Unnikrishnan M., Vimala B., Santha V. Biodiversity of tropical tuber crops in India. NBA Scientific Bulletin, 2006, no. 7, p.60.

Grech-Baran M., Sykłowska-Baranek K.,Pietrosiuk A. Biotechnological approaches to enhance salidroside, rosin and its derivatives production in selected Rhodiola spp. in vitro cultures. Phytochemistry reviews, 2015, no. 14, pp. 657-674. DOI:10.1007/s11101-014-9368-y.

Kirichenko E.V., Rudenko S.S., Baglaj B.M., Masikevich U.G. Leaf culture from in vitro propagated Rhodiolarosea. Bulletin GBS, 1994, no. 169, pp. 50-54.

Hai-jun L., Bin G., Qiong Y. et al. Tissue culture of four Rhodiola species. Acta Botanica Boreali-OccidentaliaSinica, 2006, pp. 207-210.

Tasheva K., Kosturkova G. Bulgarian golden root in vitro cultures for micropropagation and reintroduction. Central European Journal of Biology,2010, vol. 5, no. 6, pp. 853-863. DOI:10.2478/s11535-010-0092-3.

Tasheva K., Kosturkova G. Establishment of callus cultures of Rhodiolarosea Bulgarian ecotype.ActaHorticulare, 2012, vol. 955, pp.129-136. DOI: 10.17660/ActaHortic.2012.955.17.

Ishmuratova M.M. Clonal micropropagation of Rhodiolarosea L. and R. iremelicaBoriss. in vitro. Plant resources, 1998, vol. 34, pp. 12-23.

Martin J. In vitro culture establishment of Schizandrachinensis (turz.) Baill. andRhodiolaroseaL., two adaptogenic compounds producing plants. Journal of Phytology, 2010, no. 2 (11), pp. 80-87.

Yin W.B., Li W., Du G.S., Huang Q.N. Studies on tissue culture of Tibetan Rhodiolarosea. Acta Botanica Boreali-OccidentaliaSinica, 2004, vol. 24, pp. 1506-1510.

Ivashhenko A.A., KotuhovJu.A. O redkihvidahlekarstvennyhrastenijZapadno-Altajskogozapovednika // Botanicheskoeresursovedenie: dostizhenijaiperspektivyrazvitija [Rare species of medicinal plants of the West Altay reserve/ Botanical Resources study: achievements and prospects], 2000, pp. 27-28.

KotuhovJu.A., Ivashhenko A.A., LajmanDzh. Flora sosudistyhrastenijZapadno-Altajskogozapovednika [Flora of vascular plants of the West Altay reserve]. Almaty: Tethys, 2002,108 p.

Ajdarbaeva D.K., Kuz'minJe.V., Gemedzhieva N.G. ResursnoemnogoobrazielekarstvennojfloryhrebtaJuzhnyjAltaj [Resource diversity of medicinal flora mountain range Southern Altai] // Mater. nauch. konf. “ProblemyobespechenijabiologicheskojbezopasnostiKazahstana” [Proc. conf. “Problems of maintenance of biological safety in Kazakhstan”]. Almaty, 2008, pp. 82-85.

Flora Kazahstana[Flora of Kazakhstan]. Alma-Ata, AN SSR, 1958-1966, vol.1-9.

Flora Sibiri[Flora Siberia]. Novosibirsk,Nauka, 1990, vol. 1-14.

Sarasan V., Kite G., Sileshi C. Applications of phytochemical and in vitro techniques for reducing over-harvesting of medicinal and pesticidal plants and generating income for the rural poor. Plant Cell Report, 2011, no. 30, pp. 1163-1172. DOI:10.1007/s00299-011-1047-5.

Liu H.J., Xu Y., Liu Y.J., Liu C.Z. Plant regeneration from leaf explants of Rhodiolafastigiata. In vitro cellular and developmental biology. Plant, 2004, vol. 42, pp. 345-347. DOI:10.1079/IVP2006773.

Romanov G.A., Medvedev S.S. Auksinyicitokininy v razvitiirastenij. Posledniedostizhenija v issledovaniifitogormonov. [Auxins and cytokinins in plant development. Recent advances in the study of plant hormones]. Fiziologijarastenij, 2006, vol. 53, no. 2, pp. 309-319.

Tlehusezh M.A., Tjuhtenjova Z.I., Nen'ko N.I. Aktivatoryprorastanijasemjanozimojpshenicynaosnoveamidovpolizameshhjonnojaminomasljanojkisloty [Activators of germination of winter wheat seeds based amide of a poly-substituted aminobutyric acid]. Sovremennyeproblemynaukiiobrazovanija, 2015, no. 1, pp. 6-13.

Ishmuratova M.M. Biologicheskie I khimicheskieosobennosti, kulturatkaneyRhodiolarosea L. iRhodiolairemelicaBoriss. ivozmozhnostihintriduktsii v Bashkortostan (naprimere g. Ufa). [Biological and chemical characteristics, tissue culture Rhodiolarosea L. and Rhodiolairemelica and the possibility of their introduction into the Bashkortostan (for example, the city of Ufa). Kand. biol.sci.diss.]. Dis. cand.biol.nauk. Ufa, 1996, 20 p.

Tasheva K., Kosturkova G. The role of biotechnology for conservation and biologically active substances production of Rhodiolarosea: endangered medicinal species. The Scientific World Journal, 2012, vol. 3, 13 p. DOI: 10.1100/2012/274942.

György Z., Tolonen A., Pakonen M. et al. Enhancing the production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiolarosea by biotransformation of cinnamyl alcohol. Plant Science, 2004, vol. 166, no. 1, pp. 229-236. DOI: 10.1016/j.plantsci.2003.09.011.

Butenko R.G. Biologijakletokvysshihrastenij in vitro ibiotehnologiinaihosnove [Biology of cells of higher plants in vitro and Biotechnology based on their]. M., FBK-PRESS, 1999, 160 p.